Gene expression and mental disease

Gene expression and mental disease

The structure and function of an organ such as the brain depends largely upon its constituent cells and the pattern of proteins which they synthesize; this in turn results from the expression of specific genes within each cell (Milner et al. 1987). Given that all human cells contain the same genetic material, differences in cell phenotypes depend upon mechanisms which result in expression or su...

Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

deep brain stimulation and gene expression alterations in parkinson’s disease

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Deep Brain Stimulation and Gene Expression Alterations in Parkinson’s Disease

Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...